Gene Therapy: A Lipofection Approach for Gene Transfer into Primary Endothelial Cells
نویسندگان
چکیده
منابع مشابه
Gene Transfer into Sub- cultured Endometrial Cells Using Lipofection
Lipofection using the Lipofectin® reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a β-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (105 cells per 35-mm well) at the time of subculture (48 h bef...
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Lipofection using the Lipofectin reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a beta-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (10(5) cells per 35-mm well) at the time of subculture (48 h...
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The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro development of somatic cell nuclear transfer (SCNT) embryos. Green fluorescent protein (GFP) gene was used as a foreign gene and a non-transfected somatic cell was utilized as a control karyoplast. Mon...
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OBJECTIVE Progressive graft arteriosclerosis is responsible for the majority of late deaths in cardiac transplant recipients. Despite many investigations, the pathogenesis of this disease remains undetermined and its control inadequate. A somatic gene transfer during the cold ischemic time and thus before transplantation might be a new therapeutic tool. This approach allows a long incubation ti...
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Readers of Arteriosclerosis, Thrombosis, and Vascular Biology are already well aware of the high level of interest in gene therapy as a potential means of combating cardiac and vascular disease. During the last 10 years or so there have been clear demonstrations in a variety of animal models that it is possible to modulate the progress of atherosclerotic vascular disease, restenotic arterial di...
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ژورنال
عنوان ژورنال: Cell Transplantation
سال: 2002
ISSN: 0963-6897,1555-3892
DOI: 10.3727/000000002783985495